BindCraft: one-shot design of functional protein binders
Peer-reviewed| Поле | Значение |
|---|---|
| Authors | NR |
| Year | 2025 |
| Venue / status | Peer-reviewed journal; Nature (Q1) |
| DOI | 10.1038/s41586-025-09429-6 |
| URL | https://doi.org/10.1038/s41586-025-09429-6 |
| Preprint URL | NR |
| Tools | BindCraft |
Задача и claimed novelty
| Поле | Значение |
|---|---|
| Main biological task | Target-conditioned de novo non-antibody protein binder design |
| Claimed novelty | NR |
Архитектура
| Поле | Значение |
|---|---|
| Model architecture | AlphaFold2 backpropagation/hallucination + MPNN redesign + PyRosetta filtering |
| Input representation | NR |
| Encoder / decoder | NR |
| Generative state | Binder sequence and designed target–binder complex |
| Conditioning | Target chain, hotspot/interface residues, binder length and filter thresholds |
| Sampling | NR |
Training
| Поле | Значение |
|---|---|
| Objectives / losses | NR |
| Dataset | NR |
| Preprocessing | NR |
Inference
NR
Baselines и retrospective benchmarks
| Поле | Значение |
|---|---|
| Baselines | NR |
| Retrospective | NR |
Prospective experimental validation
| Поле | Значение |
|---|---|
| Prospective | Nanomolar binders and functional examples; general binders |
| Tested designs | Multiple targets; reported 10–100% task-dependent experimental success rates |
| Success criterion | Nanomolar binders and functional examples; general binders |
| Main results | Nanomolar binders and functional examples; general binders |
Limitations
Not antibody-specific; target structure/hotspot selection and AF2 optimization bias matter; integrated confidence is not experimental affinity.
Что статья показывает
- Nanomolar binders and functional examples; general binders
Чего статья не показывает
- Predicted confidence, likelihood, pLDDT, docking or developability scores are not experimental affinity.
Размеченные утверждения
| Утверждение | Тип | Локатор |
|---|---|---|
| BindCraft: one-shot design of functional protein binders | Interpretation | DOI/URL from landscape matrix |
| Publication status: Peer-reviewed journal; Nature (Q1) | Author-reported | landscape matrix, publication status column |
Review queue
- Bibliographic title must be verified against the PDF.
Source-grounded разбор статьи
Исследовательский вопрос
[MATRIX]Target-conditioned de novo non-antibody protein binder design
Утверждения и поддержка evidence
[MATRIX]Claimed novelty: NR
[INTERPRETATION]Статья не «доказывает» общее преимущество метода вне своего экспериментального дизайна.
Что представленный evidence не поддерживает
- Predicted confidence, likelihood, pLDDT, docking or developability scores are not experimental affinity.
Ограничения, признанные авторами
[MATRIX]Not antibody-specific; target structure/hotspot selection and AF2 optimization bias matter; integrated confidence is not experimental affinity.
Дополнительные ограничения интерпретации
NRПока Methods/SI не прочитаны, нельзя утверждать split leakage, точные гиперпараметры и полноту ablation.