Framework humanization
Практический сценарий
Есть non-human antibody или CDRs; нужно human-like framework.
Вход
- paired VH/VL or CDR sequences
Pipeline diagram
Framework proposal
→Humanness scoring
→Binding retention tests
Mermaid diagram
flowchart TD
A[Parent VH/VL or CDRs] --> B[Humanization generator]
B --> C[Humanness / pairing scores]
C --> D[Binding retention assays]
D --> E[Immunogenicity still unproven]Стадии pipeline
| Стадия | Архитектурный класс | Что происходит | Генерация или оценка | Generator | Промежуточный объект | Альтернативы |
|---|---|---|---|---|---|---|
| Framework proposal | Classifiers or sequence diffusion | Предлагаются humanized sequences | generation | humatch | Humanized V-regions | HuDiff, HuAbDiffusion |
| Humanness scoring | Nativeness/humanness | Оценка human-likeness | evaluation | NR | Scored variants | AbNatiV / BioPhi / OASis |
| Binding retention tests | NR | Проверка, что CDRs всё ещё bind | experiment | NR | Retained binders | NR |
Hardware и runtime
Humatch: code + web service, typically lightweight. HuAbDiffusion: commercial service, source NR.
Evidence level
Humatch retrospective. HuDiff limited prospective preprint. Humanness ≠ immunogenicity.
Точки отказа
- Assume immunogenicity reduction.
- Skip binding assays.
- Use HuDiff-Nb as de novo binder tool.
Критерии решения
- Paired VH/VL available?
- Need VHH humanization?
- Need open source?